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By T.K. Ghose ,A. Fiechter, N.Blakebrough

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C u l t u r e Fluids C o n t a i n i n g E x t r a c e l l u l a r P o l y s a c c h a r i d e s The rheological properties of a culture fluid containing an extracellular microbial polysaccharide is controlled by the concentration and molecular properties of the dissolved polysaccharide. While the cells do have some influence, there is evidence that it is relatively minimal as illustrated in Fig. 19 for Xanthomonas campestris broths before and after clarification [56]. Air bubbles may also effect rheological measurements.

Viscosity increases monotonically with reaction time. 2. Nature of rheological behavior not given. not given Brookfield No 1. Medium contains dextran. 2. Viscosity measurement provides excellent measure of dextranase production. 3. Nature of rheological behavior not discussed. 0 Brookfield No 1. Viscosity increases monotonically with reaction time. 2. Nature of rheological properties not discussed. 0 1 RPM (~ not given) No. Brookfield; Brookfield-Wells cone and plate 1561 (whole broth and filtered broth) X.

Mixing time correlations for S. niveus culture fluid for various turbine impeller configurations 1991 Technical Aspects of the RheologicalProperties of Microbial Cultures 39 are in good agreement qualitatively with the original Norwood-Metzner [84] correlation although they differ quantitatively. The discrepancies might be due to aeration effects as demonstrated by the work of Blakebrough and Sambamurthy [98]: Wang and Fewkes [99] did not specify whether mixing times were determined under aerated or non-aerated conditions nor did they mention the extent of gas hold up.

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