Download Analytical Biotechnology by C. van Dijk (Eds.) PDF

By C. van Dijk (Eds.)

ANABIOTEC '92 considering the extra integration of biotechnology and analytical chemistry. the result of this symposium basically validated mammoth growth might be mentioned within the program of either traditional and new analytical options, the latter basically in accordance with normal analytical instruments equivalent to biomolecules. the most issues coated in this assembly are fermentation tracking, chromatography, instrumental research, biosensors and bioanalysis

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Chim. Acta 279 (1993) 27-37 34 (v/v) ethanol solution for several days. However, no visual deteriorations or dissolution tendencies or similar could be observed. This indicates that one might use the cellulose membrane also for sampling purposes in the FAM. However, the polymer-based membranes are of greater mechanical strength and were therefore chosen. Amino acid recovery in an ethanol fermentation substrate and a penicillin fermentation broth The experiments described above provided valuable information on how well known interferences affect membrane transport using TFF for carbohydrates in defined model solutions.

On-line measurement usually requires the use of a filtration interface which allows direct sample introduction into an analytical system by removing interfering broth components. Flow-injection analysis (FIA) is the most frequently applied technique for the on-line monitoring of chemical variables and is normally performed in combination with a microfiltration interface for the removal of cells. Although FIA is a rather simple and fast technique, it is difficult to determine several compounds simultaneously and for accurate quantification selective enzymatic or chemical reactions are needed.

Suboxydans converts glucose into gluconic acid by means of glucose dehydrogenase. Between 17 and 19 h, 750 ml of a 300 g l" 1 aqueous glucose solution were added to the fermenter. In this period the glucose concentration therefore sharply increased, whereas the gluconic acid concentration slightly decreased because of dilution of the fermentation broth. By the action of gluconate dehydrogenase gluconic acid can be further transformed into 2-keto- and 5-ketogluconic acid [19]. Figure 5 shows that small amounts were present in the broth at the end of the fermentation, as can also be seen from Fig.

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