By Li Zhensheng (auth.), Chongbiao You, Zhangliang Chen, Yong Ding (eds.)
The First Asia --- Pacific convention on Agricultural Biotechnology was once held in Beijing, China on 20-24, August, 1992. Over part the inhabitants on the earth is within the Asian and Pacific sector. With an expanding inhabitants and lowering farming lands, you will need to increase agricultural biotechnology for development of the productiveness, profitability and balance of the farming method. The Conference's major ambitions have been to compile scientists operating in several fields of agricultural biotechnology to stimulate dialogue in this very important approach and to have an appraisal of the newest experiences bearing on genetic manipulation of vegetation, plant mobilephone and tissue tradition, plant gene law, plant-microbe interplay, animal biotechnology and so forth. The convention used to be attended by way of 391 scientists from various nations and areas. This quantity provides the contributions of the lectures and a specific variety of posters, that are an up to date account of the nation of information on agricultural biotechnology. The booklet offers a important reference resource not just for experts in agricultural biotechnology, but in addition for researchers engaged on comparable elements of agronomy, biochemistry, genetics, molecular biology, microbiology and animal sciences. it truly is with nice excitement to recognize the contributions of the authors in assuring the steered ebook of this quantity. we might additionally expand our honest thank to Kluwer educational Publishers for the e-book of those proceedings.
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Additional info for Biotechnology in Agriculture: Proceedings of the First Asia-Pacific Conference on Agricultural Biotechnology, Beijing, China, 20–24 August 1992
Plant Cell Tissue and Organ Culture 18: 47-53. Smith, J. , M. R. Krauss, C. Borkird and Z. R. Sung. (1988) A nuclear protein associated with cell divisions in plants. Planta 174: 462-472. Choi, J. , L. S. Liu, C. Borkird and Z. R. Sung. (1987) Isolation of cDNA clones for rare embryo-specific antigens in carrot cell cultures. Proc. Natl. Acad. Sci. USA 84: 1906-1910. , J. H. Choi, Z. Jin, G. Franz, P. Hatzopoulos, R. Chorneau, R. Bonas, F. Pelegri and Z. R. Sung. (1988) Developmental regulation of embryonic genes in plant.
We screened the cDNA libraries differentially to isolate the hypocotyl- or root-specific cDNAs. Finally we isolated 2 cDNAs, CAR 3 and CAR 4, which were specifically expressed in hypocotyls, and other 2 cDNAs, CAR 5 and CAR 6 specifically expressed in roots. Expression of these 4 cDNAs was investigated in Phase 1-3 by Northern hybridization. Relative expression of CAR 4 and 5 increased after globular embryos and that of CAR 6 increased a little later (after heartshaped embryos), 'while CAR 3 was expressed earlier (before globular embryos), that is, in Phase 2.
The heterokaryotic fusion frequency was between 5 to 20% of the surviving protoplasts depending of the genotypes involved in the fusion. carinata protoplasts regeneration to complete plantlets. Somatic hybrids grew in cultures vigorously except in one combination: Camelina sativa + B. carinata. sativa in slow growth. Also, it could not be rooted to get hybrid plants. Morphology and Cy~ology All somatic hybrids were intermediate between the parents in morphological characteristics. Cytology was studied in six combinations.